Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative detected hnRNP A2B1 in human tonsil, human small intestine, and human uterus tissues.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected hnRNP A2B1 in 10 µg of NIH/3T3.

This mouse monoclonal Anti-hnRNP A2B1, clone 12B12.2 Antibody, Cat. No. MABF1098 is tested in Immunohistochemistry (Paraffin) and in Western Blotting for the detection of hnRNP.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Heterogeneous nuclear ribonucleoproteins A2/B1 (UniProt: P22626; also known as hnRNP A2/B1) is encoded by the HNRNPA2B1 gene (Gene ID: 3181) in human. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. It contains 2 RNA recognition motifs. Through alternate splicing two isoforms are generated. Isoform 1 (B1) is localized in cytoplasmic mRNP granules containing untranslated mRNA and in exosomes. It is not found in the nucleolus. Isoform 2 (A2) is predominantly nucleoplasmic, however it is also found in the cytoplasm of cells in some tissues. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. The hnRNP proteins are involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons and they acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm. They are also reported to specifically bind single-stranded telomeric DNA sequences, thereby protecting telomeric DNA repeat against endonuclease digestion.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human heterogeneous nuclear ribonucleoproteins A2/B1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected hnRNP A2B1 10 µg of HeLa cell lysate.

Specificity

Clone 12B12.2 specifically detects heterogeneous nuclear ribonucleoproteins A2/B1. it targets an epitope within 15 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed; 37.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).